Yeast Protocols

• Genomic  DNA isolation from yeast   
• Yeast DNA Preparation by rapid glass bead method
• Yeast Genomic DNA Isoaltion   
• Yeast quick plasmid DNA Preparation  
• Isolation of yeast DNA by smash and grab method    
• Yeast Genomic DNA Extraction 2-D Gels     
• Preparation of Yeast DNA Embedded in Agarose Plugs  
• Yeast Genomic DNA Miniprepartion  
• Yeast Chromosomal  DNA Preparation    
• Yeast DNA Minipreparation   
• High Molecular Weight Yeast Liquid DNA Preparation   
• Restriction Digests of High Molecular Weight Yeast DNA    
• Isolation of Yeast Genomic DNA    
• DNA Isolation from Yeast  
• Yeast RNA Preparation
• Yeast RNA Minipreparation
• Streaking Yeast Stocks  
• Storage of Yeast Stocks  
• Yeast Sporulation 
• Yeast Sporulation in Liquid Media  
• Yeast sporulation Protocol 
• Yeast Complete Media 
• Yeast Media, Solutions and Stocks       
• Yeast Media  
• Yeast Transformation  
• Yeast Transformation  
• Large-Scale Yeast Transformation  
• Yeast Transformation  
• Transformation of Yeast by Lithium Acetate  Method  
• A Rapid method of Yeast Transformation 
• Yeast Transformation  
• Fission Yeast Transformation  
• Multiwell Transformation of Yeast   
• Quick Yeast Transformation  
• Competent Yeast Preparation  
• High efficiency yeast Transformation with DMSO or LioAc 
• Genome-wide Gene Expression Analysis  
• Restriction Digests of High Molecular Weight Yeast DNA   
• Preparation of Yeast Cell for Immunoeletron Microscopy  
• Yeast Immunofluorescence with Methanol/Acetone Dehydration  
• Yeast Immunofluorescence protocol
• Staining of yeast by Rhodamine-Phalloidin/Calcofluor 
• Flow Cytometry of yeast   
• Vectorette PCR of Yeast DNA
• Yeast Actin Capture Assay  
• Determination of replication Timing of yeast by Transient Hemimethylation 
• Determination of replication Timing of yeast by Density Transfer       
• Determination of replication Timing of yeast by Comparative Hybridization  
• UV Mutagenesis   
• EMS Mutagenesis     
• EMS mutagenesis of yeast     
• Generating a Temperature Sensitive Allele    
• Confirmation PCR  
• Plasmid Rescue from Yeast from Liquid Culture and Clony Rescue  
• Yeast Colony PCR  
• Confirmation PCR  
• Direct PCR from Whole Yeast Cells  
• ß-GAL Filter Assay   
• ß-gal liquid assay  
• Pheromone Halo Assay    
• ß-gal Lift method     
• Handling and using insertion libraries  
•  Yeast Protein Preparation
• Whole Cell Extracts
• Quick Total Yeast Protein Isolation for SDS-PAGE Gels  
• Yeast Protein Preparation for SDS PAGE and Western (rapid)  
• Yeast Protein Preparation for SDS PAGE and Western  
• Sucrose Density Gradient Fractionation of Yeast Membranes
• Protein Extraction from S. cerevisiae  
• Extracts for Yeast Westerns
• Yeast Protein Isolation  
• Yeast Nuclear Extract Prep (small scale)  
• Yeast Whole Cell Extract (small scale)  
• Performing a Hunt by Interaction Mating
• GST Fusion Protein Purification from Yeast  
• Two Hybrid System Transformation  
• Purification  of MBP (maLTose-Binding Proteins) Fused Proteins  
• Purification of His-tagged proteins from Sf9 cells under native conditions   
Purification of GST Fused Proteins