Cell biology

Transgenics Protocols

Microinjection of Single Cells in Culture
Preparation of Microinjection Pipettes
Embryo Manipulation Pipettes (Flushers etc.)
Micropipette Beveling
Preparation of DNA for Microinjection
Preparation of DNA for Microinjection
DNA Purification for Transgene Microinjection
DNA Purification for Microinjection
DNA Ultrapurification for Microinjection
DNA Preparation for microinjection or electroporation
BAC DNA Purification for Microinjection
DNA Pronuclear Injections
Microinjection of Single Cells in Culture
ES Cell Pipette Manufacture
DNA Purification for Electroporation
Transgenic Outline
Harvesting of Cells for Blastocyst Injection
Vasectomy of Male Mice
Vasectomy of Mouse
Collection of Morulae and Earlier
Aggregation of ES Cells and Eight Cell Stage Embryos
One cell embryo transfer into pseudopregnant recipient female mouse
Blastocyst Transfer
Sending Sera to Testing for Mouse Pathogens
Superovulation of Mice
Flushing Out of Blastocysts
Alizarin Red S (Bone) and Alcian Blue (Cartilage) Staining of Cleared Skeletons
PCR Genotyping of Embryos and Blastocysts
PCR Genotyping from the Embryo Yoke Sac
ß-Gal Staining of the Murine Embryo
b-gal Solution Assay : for quantitative analysis
Whole Mount X-gal Histochemistry of Transgenic Animal Tissues and Cultured Cells
Electroporation of ES Cells and Isolation of H/R Clones
Electroporation of ES Cell
ES / MEF Cell Culture and Electroporation of Targeting construct
Blastocyst Embryo Transfer into Pseudopregnant Recipient Female Mouse
Transgenic Screening
Genotyping Transgenic Mice by PCR
Yolk Sac DNA Preparationor Southern or PCR
DNA Purification for PCR only from yolk sacs, toes, or 2 mm Tail
Mouse Tail Digestion Procedure
PCR of DNA from Ear Clip Tissue
Genomic Southerns of DNA from Tail Clips
Isolation of DNA from Mouse Tail Biopsies
Genomic DNA preparation
DNA Isolation from Murine Tails without Phenol Chloroform
Non-organic Isolation of Tail DNA
DNA Extraction from Tail Biopsies
Mouse Tail (or organ) Biopsy DNA Extraction
Isolation of DNA from Mouse Tail Biopsies
Preparation of Mouse Tail DNA for Dot Blots or PCR
Preparation of Copy Standards for PCR Genotyping
PCR Analysis of Tail DNA
LacZ Staining of Embryos
Whole Mount LacZ Staining
Whole Mount Human Placental Alkaline Phosphatase Staining
Whole Mount Immunoflurescentstaining
Whole Mount RNA in situ Hybridization
Immunocytochemistry Staining of Embryos
X-gal & AP Staining Protocol
RNA Whole Mount In Situ Hybridization
Timeline for Transgenic Mouse Production/Analysis
Transgenic Strain Staining Assay
Mating Transgenic Mice