Transformation

• Preparation of Competent Cells
• Preparation of Competent Cell by rubidium chloride method
• Preparation of Competent E. coli Cells
• Preparation of Competent Cell
• One-step competent E. coli cells
• Preparing of  Bacterial Cells for Electroporation

• Electroporation Competent E.Coli Preparation
• Transformation of Bacteria
• Transformation  of E. Coli
• Transformation of E.Coli, M13 Cloning 
• Quick Transformation method
• Transformation of Competent Cells (RbCl2 Method)
• Transformation of E. coli
• Standard Transformation Protocol
• Protocol for Transforming Competent E. coli Cells
• Transformation of Aspergillus niger
• Transformation of Aspergillus nidulans  
• Large-scale transformation procedures
• Transformation of E. coli by Electroporation
• Transformation of E. Coli by Electroporation
• Preparation of DNA for Electroporation
• Electroporation of P. Aeruginosa
• Transfer of DNA from Bacterial Colonies to Nylon Membrane or Nitrocellulose
• Screening clone by PCR
• Slot Lysis
• Bacterial Colony PCR   
• Toothpick Plasmid Assay
• PCR Screening of Transformants
• Bacteria Storage
• In situ screening of bacterial colonies
• Calculating Transformation Efficiency

Transfection

• Transfection of Mammalian Cells Using Lipofectamine

• Transfection of Mammalian Cell by Calcium Phophate method
• Transfection of HeLa Cells
• Eukaryotic Cell Transfection
• Selection of Transfected Suspension Cells
• Transfection of Mammalian cell
• Eukaryotic Transfections with Cationic Lipid Reagents
• Transfection of Mammalian Cell by Electroporation method
• CAT Assay
• ß-Galactosidase assay for the in situ Expression
• Luciferase assay reagents
• Beta-Galactosidase Enzyme Assay . 
• ß-gal staining of eukaryotic cells in vitro