Plant Molecular Biology Protocols

• Rice
• Arabidopsis
• Cotton
• General Protocols

Rice

• Preparation of DNA from Single Rice Seedlings
• Isolation of RNA and DNA from Rice Seedling Mitochondria
• A simple screning method for transgenic rice tissue based on PCR
• Non-aqueous Method for Purification of Cytoplasmic DNA from Individual Rice Plants for RFLP Analysis
• Enhancement of Division Frequency of Protoplasts and Plant Regeneration in Rice
• Isolation of RNA and DNA from different rice tissues
• Improved histochemical staining for beta-D-glucuronidase activity in monocotyledonous plants
• A rapid, reliable method for preparing somatic chromosomes of rice
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Arabidopsis

• Arabidopsis DNA Purification from Nuclei
• Arabidopsis Seed Collection
• Arabidopsis Transformation
• Arabidopsis Vacuum Infiltration
• Arabidopsis transformation (infiltration)
• How to grow Arabidopsis
• Arabidopsis seed sterilization
• Arabidopsis EMS mutagenesis of seeds
• Arabidopsis quick genomic DNA prep for PCR

Cotton

• Collecting Cotton Tissue
• Cotton DNA Isolation
• RNA Extraction from Cotton
• Agrobacterium-mediated Transformation of Cotton

General Protocols

• Agrobacterium competent cells
• Agrobacterium electroporation
• Agrobacterium miniprep
• Agrobacterium miniprep (phenol free)
• Tissue Lyophilization
• Plant DNA Extraction Protocol 
• Plant DNA Extraction Protocol 
• Plant Nuclei DNA Extraction Protocol 
• Plant Mitochondrial DNA Extraction Protocol 
• Plant Chloroplast DNA Extraction Protocol
• Isolation of Genomic DNA from Azorhizobium caulinodans ORS571
• DNA Extraction from ORS571
• DNA Extraction Procedure for Rhizobium spp.
• Lessons In Paranoia
• Plant RNA from Potato
• Tissue Lyophilization
• cDNA probes
• Colony hybridization
• Concanavalin A Blots
• DNA PAGE
• E coli competent cells (CaCl2)
• E coli competent cells (RuCl2)
• E coli transformation
• End labelled probe preparation
• Filter washing
• Fluorography of PAG
• Fluorometer/Luminometer Wallac Victor II
• Footprint with DNAse1
• Gelshift
• Genomic DNA from cereal leaves
• GST fusion protein purification
• GUS histochemical assay
• GUS & LUC bombardment assay
• GUS luminometric assay
• HIS-tagged protein purification
• Hormone treatments
• Hormones & inhibitors
• Hybridization selection of cloned DNA
• Immunoprecipitation from in vitro translations
• Isotachophoresis probe isolation
• In vitro transcription
• In vitro translation
• Isolation of Lambda DNA by plate lysate
• Lambda lysate
• Leaf PCR
• Maxam Gilbert sequencing
• Microsomal preps for membrane proteins
• Miniprep - alkaline lysis
• NEpHGE Non-equilibrium pH gel electrophoresis
• Northern blot in formaldehyde gel
• Nuclear extract preparation
• Nuclei prep
• Nuclear prep from protoplasts
• Oligo dT cellulose purification of mRNA
• Oligo probe hybridization
• Oligonucleotide probe preparation
• Phage strains
• Plasmid prep with CsCl
• Primer extension with synthetic oligonucleotide
• Progeny analysis of plants
• RNA extraction in GT w/ CsCl spin
• RNA large scale from starchy tissue
• RNA miniprep 1
• RNA miniprep 2
• RT-PCR
• SDS-PAGE for proteins
• Seed sterilization for cereals
• Silver staining of proteins in PAG
• Soluble protein extraction
• Southwestern blotting
• T4 polymerase fill in rxn

• Triparental Mating 

• Triparental Mating 

• Western blotting
• Whole cell extracts