Polymerase Chain Reaction (PCR) Protocols

 

• Standard PCR Protocols
• RT PCR
• PCR Modifications
• Miscellaneous

Standard PCR Protocols

• Know about PCR?  

• PCR Guide  

• Basic PCR Guide  

• PCR and multiplex PCR guide  

• Standard PCR Protocol  

• PCR Manual   

• Amplification of Genomic DNA by PCR  

• PCR Trouble Shooting Guide

• Calculations of  Concentrations for PCR 

• Core sample PCR 

• DNA Amplification in the Presence of Agarose 

• RT PCR

• RT-PCR Protocol  

• Reverse Transcriptase PCR  

• RT-PCR  

• cDNA Synthesis and Amplification of POLY(A) mRNA by RT-PCR  

• Nested RT-PCR From Paraffin Section  
  

• Competitive RT-PCR  

• Competitive Quantitative RT-PCR  

• Semi-Quantitative RT-PCR  

• RT In Situ PCR  

• In Situ RT-PCR  

• Reverse Ligation Mediated RT-PCR

• PCR Modifications

• Touchdown PCR 

• "Hot Start" PCR 

• In Situ PCR Cookbook  
• In Situ PCR on fixed tissues or cells

• In Situ PCR on Plant Materials  

• RT In Situ PCR  

• In Situ PCR On Plant Tissues

• Asymmetric PCR for ssDNA Production

• Inverse PCR and Cycle Sequencing Protocols

• Inverse PCR for isolation of DNA fragment

• Inverse PCR

• Vectorette PCR of Yeast DNA  

• PCR-Elisa

• PCR of Blood, Hair or Small Tissue Samples
• PCR from Tissue without DNA extarction
• PCR for  Biotinylation of Probes
• Colony PCR
• Colony PCR for E.coli or Yeast

• Direct PCR from Whole Yeast Cells

• Degenerate PCR

• Degenerate PCR Guide

• Solid Phase DNA Amplification  

• Site-directed Mutagenesis using PCR

• PCR Products Labeling

• Purification and Sequencing of PCR Product
• PCR Product Sequencing
• Purification of PCR products from 96 well plate
• PCR of M13
• Gene Disruption by Fusion PCR
  
• PCR amplification of rRNA gene fragment.
• PCR for Radiation Hybrid Mapping  
• PCR Based Gene Deletion  
• Single Tube Confirmation PCR Protocol  
• Non-organic Isolation of Tail DNA and PCR  
• Extraction of DNA from Blood for PCR  
• attB Adapter PCR Protocol  
• Miscellaneous

• Agarose Gel Electrophoresis of PCR Products  

• Purification of PCR products using Solid-Phase Reversible Immobilization Technique  

• PCR Products Labeling  

• PCR Products Purification  

• PEG Purification of PCR Products 
• DNAse I Protocol: The Use of DNAse I Prior to PCR
• Purification and Sequencing of PCR Product 
• PCR Product Sequencing  
• Digestion of PCR Products 
• 96 Well Plate Purification of PCR products
• Blunt End Cloning PCR Products  
• Cleavage Efficiency Close to the Termini of PCR Fragments  
• DNA Contamination in RT-PCR  
• Extraction of PCR Oil Using Chloroform

• Yeast Colony PCR  

• Confirmation PCR  

• Direct PCR from Whole Yeast Cells   
• Purification and concentration of DNA from PCR product