Drosophila Protocols

• Fly food recipe
• Quarantine of new stocks
• Fly Husbandry  
• Media recipies  
• Fly Culturing
• Embryo Fixation  
• Immunostaining Drosophila Embryos  
• Embryo antibody stains  
• Fluorescent in situ hybridization  
• RNA In situ protocol  
• In situ RNA stains with digoxygenin 
• In situ/Antibody double labeling in embryos 
• Dissection and staining of larvae & pupae 
• Antibody staining of imaginal discs
• Pupal staining with BrdU.
• In situ RNA hybridization for Imaginal Discs.
• Antibody/In situ double staining of imaginal discs.
• Xgal staining of discs  
• Drosophila Head Collection
• Drosophila DNA Preparation
• Drosophila RNA Prep
• Immunohistochemistry on Drosophila Brains
• Microscopic Analysis of the Adult Retina
• Adult Head Frozen Tissue Sections
• B-Galactosidase Activity Staining
• Antibody Staining
• Antibody Staining of Adult Retina
• Chromosome In Situ Hybridization Using Biotin Labeled Probes
• Whole Mounts of Pupal Retinas
• Cuticle preparations
• The Developmental Studies Hybridoma Bank
• The Antibody Resource Page
• X Ray Mutagenesis
• Embryo Transformation
• Generating Somatic Clones by FRT Recombination
• Somatic Mosaic Analysis in the Eye
• Drosophila DNA microarrays  
• Far Western blotting
• Rapid Fly DNA preparation(for PCR).
• Vector maps at FlyBase.
• Drosophila vector sequences  
• Maps of P1 vectors used in the Drosophila genome project
• Maintenance of imaginal disc cell lines
• Drosophila Cell Lines
• Maintenance of the cl8+ (clone 8) and other imaginal cell lines.