DNA  Protocols

DNA Extraction from Microorganisms/tissues
DNA Extraction from Plants or Fungi
DNA Purification
Recovery of DNA From the Gel
Gel Electrophoresis

• DNA extraction from Bacteria

• >DNA extraction From Bacteria (Triton Prep)

• Genomic DNA isolation from P. aeruginosa

• Extraction of DNA from Gram-positive Bacteria

• Extraction of Total Genomic DNA from Microarthropods

• DNA extraction from Tissue

• DNA extraction from Mouse Liver

• Genomic DNA Isolation from Blood  

• Manual Isolation of Human DNA from PBMC or Whole Blood     

• Procedure for Human DNA Extraction through Salting out method    

• Silica Gel method of DNA extraction from the Whole Blood

• DNA extraction from Blood for PCR reactions

• Solid-Phase Reversible Immobilization (SPRI) Technique for DNA Purification

• Phenol/Chloroform Extraction of DNA

• Mouse Genomic DNA Isolation

• Phenolic extraction of DNA  

• Isolation of High Molecular Weight Plant DNA

• A Simple Method of Plant Genomic DNA extraction

• Isolation of Genomic DNA From Maize  

• Genomic Plant DNA Extraction     

• Isolation of Plant Geneomic DNA (CTAB metod)

• Rapid DNA Extraction and Purification  

• Half Seed DNA Extraction Protocol

• Preparation of Megabase-size DNA

• Mini DNA extraction from wheat leaves for PCR   

• Large Scale Monocot DNA Isolation  

• Extraction of Chloroplast and Nuclear DNA

• DNA Purification-Modified CTAB Procedure

• Single-Fly DNA Preparations for PCR

• Fungal Genomic DNA Extraction    

• DNA Isolation from Fungus

• General Protocol for Precipitation of DNA with Sodium Acetate and Ethanol

• Ethanol Precipitation of DNA

• DNA/RNA precipitation

• Concentration of DNA by ethanol precipitation

• Ethanol Precipitation of DNA

• DNA Precipitation

• Fragment purification on Sephacryl S-500 spin columns

• DNA Extraction from Agarose Gel

• Electroelution of DNA Fragments From Agarose into Dialysis Tubing   

• DNA Fragment Purification from Agarose or Acrylamide   

• Isolation of Restriction Fragments from Agarose Gels by Collection onto DEAE Cellulose   

• Oligo Purification on Acrylamide Gels

• DNA Isolating From Low Melting-Temperature Agarose

• Elution of DNA fragments from agarose    

• Glass_Powder Purification of_DNA from Agarose Gel

• DNA Isolation from Agarose Gels with DEAE Paper 

• Gel Purification through B Agarase

• Oligonucleotide purification by PAGE and DEAE

• DNA Extraction from Agarose  

• DNA Isolation from Agarose Gel Using NaI

• DNA Fragment Purification from Agarose /Acrylamide  

• Preparation and Casting Agarose Gels

• Agarose Gel Electrophoresis

• DNA Mobility in Gels

• Trouble Shooting DNA Agarose Gels

• Denaturing Gradient Gel Electrophoresis (DGGE)

• Standard 2-D Gel of DNA Replication Intermediatess    

• Quantification  of DNA  

• DNA concentration assay with fluorescent dye
• Estimation of DNA Concentration  
• Spot Test
• Quantification of Nuclear Acid and protein  
• Quantitation of DNA for Sequencing Using Fluorometer