<Bioinformatics>< Sequence Analysis Tools>

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Sequence Database
Sequence Similarity
Alignment Tools
Protein Analysis Tools
DNA / RNA Analysis
Translation Tools
Primer Designing
Restriction Analysis
Microarray

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 Sequence Database

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NCBI - National Center for Biotechnology Information (GenBank)

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EBI - European Bioinformatics Institute (EMBL)

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EMNEW - Index of New EMBL Sequences ( EBI)

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DDBJ - DNA Data Bank of Japan

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SWISS-PROT- Protein sequence database

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SWISSNEW - New SwissProt Sequence  Entries @ EBI

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PIR - Protein Information Resource

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MIPS - Munich Information centre for Protein Sequences               

Sequence Similarity  Search

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BLAST -Basic local alignment search tool @ NCBI 

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Blast Search @EBI 

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Blast Search @Expasy

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Blast Search @ISREC

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Blast Search @Pasteur Institute , France

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PSI BLAST -Position Specific Iterated Blast search @ NCBI

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C. elegans Blast Server @ Sanger Centre: Sear

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FASTA Fasta or fastx search @ EBI

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BLAST Microbial Genomes -Genomic sequences search @ NCBI

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MPsrch -Smith-Waterman algorithm-based search

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BLASTPAT - BLAST-based Pattern Database Search

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FASTPAT - FASTA-based Pattern Database Search

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SectionSearch -FastA or TFastA search against predefined  Sequence database

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WU-BLAST Archives - Washington University School of Medicine, St. Louis

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UNIBLAST - BLAST Against Organism specific Sequences

bullet THC-Blast - Tentative Human Consensus Sequences BLAST

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Sequence Alignment

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ClustalW- Multiple Sequence alignment @ EBI, UK

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ClustalW - Multiple Sequence alignment @ Baylor College of Medicine, USA

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ClustalW -Multiple sequence alignment @  WUSTL, USA

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Match-Box Multiple sequence alignment  Tool @ Belgium

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Align -Pairwise sequence alignment

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MAP -Multiple alignment of (long) sequences without penalizing large gaps

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CINEMA- Color INteractive Editor for Multiple Alignments at BCM group, UK

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AMAS -Analyze multiple aligned sequences at Oxford University, UK

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BEAUTY - BLAST Enhanced Alignment Utility

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BOXSHADE - Printing and shading of multiple alignments

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Alignment Utilities Alignment and create the consensus alignment

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USC Sequence Alignment Server 

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MSA 2.1 -Optimal multiple alignment using the Carrillo-Lipman method

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SIM4 - Align cDNA and Genomic DNA

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Wise2 -Align  protein  genomic sequence to predict a gene structure

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PipMaker - Alignments of similar regions in two long DNA sequences

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T-COFFEE - Multiple sequence alignment  tool, CNRS France

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VISTA - Align and find  conserved regions in Genomic sequences

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SAM - Sequence Alignment and Modeling System @ UCSC                              

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Protein Analysis Tools

bullet 3DB Browser -Structure database browser @ Weizmann Institute of Science, Israel
bullet 3DCrunch -Database Browser of modeled Swiss-Prot proteins at ExPASy, Switzerland
bullet 3D-Jigsaw -Comparative modeling server
bullet AAT- Analysis and Annotation Tool for finding genes in genomic sequences 
bullet ASC -Analytic Surface Calculation of PDB molecules @t EMBL, Heidelberg)
bullet BIND -Biomolecular Interaction Network Database
bullet BLOCKS Search -Search a protein against BLOCKS database
bullet BTPRED -Prediction of beta-turns
bullet CD-Search-Search a protein against CDD domain database
bullet ChimePlugin for structure view
bullet Cn3D-Plugin for structure view for structure @ NCBI
bullet COGnitor - Search a protein against the COG database
bullet eMOTIF Search -Assign putative function to new proteins by sequence comparison with IDENTIFY motif database.
bullet Coils -Prediction of coiled Coil regions by  Lupas' method
bullet CPHmodels-Structure prediction by comparative homology modeling
bullet DAS -Prediction of transmembrane regions in prokaryotes using the Dense Alignment Surface method @ Stockholm University
bullet DGPI - Prediction of GPI-anchor and cleavage sites
bullet DIP -Search Database of Interacting Proteins
bullet DOMO - Homologous protein domain families database
bullet DOMPLOT -Structural domain organization annotated by ligand contacts.
bullet eMATRIX Search - Predict function by sequence analysis using minimal-risk scoring matrices.
bullet eMOTIF Maker - Generate motifs  describing protein families or superfamilies
bullet FAMS -Fully Automated Modeling Service
bullet FingerPRINTScan -Search a protein sequence against protein motif fingerprints database
bullet Helical Wheel - Represents alpha-helical peptides
bullet HMMTOP - Prediction of transmembrane helices and topology of proteins
bullet HNN - Hierarchical Neural Network secondary structure prediction
bullet Hydropathy plots - Plot Hydropathy
bullet InterPro Search -Search a protein against the integrated protein domains and functional sites
bullet JPred - Protein secondary structure prediction
bullet LIBRA - LIght Balance for Remote Analogous proteins: search compatible structure of a target sequence by threading
bullet List of comparative modeling tools
bullet LOCK Compare 3D structures of query and target proteins and  their  visualization of  superposed
bullet Modules -Mobile protein domains database
bullet MolSurfer -Calculate and navigate protein-protein interfaces
bullet MultiCoil -Prediction of coiled coil regions as dimeric or trimeric assemblies
bullet NetOGlyc - Prediction of O-glycosylation sites in mammalian proteins
bullet NetPhos -Prediction of phosphorylation sites in eukaryotic proteins
bullet nnPredict -Protein secondary structure prediction.
bullet PAA - Protein Annotator's Assistant
bullet PairCoil -Prediction of coiled Coil regions.
bullet PFSCAN -Protein search against different profile databases
bullet PPSearch -Search a protein sequence against prosite  pattern database
bullet Predator -Secondary structure prediction from single or multiple sequences at EMBL, Heidelberg
bullet Predicting Protein 3D structure based on homologous sequence search
bullet PredictProtein -Protein secondary structure prediction at EMBL
bullet PROCAT - Search a protein structure agains the procat database of 3D enzyme active site templates
bullet ProDom - Protein domain database
bullet ProNet Online -Database of interacting proteins
bullet PROSCAN -Search a protein sequence against prosite pattern database
bullet ProtColourer - Draw colour-coded representation of an amino acid sequence
bullet ProTherm -Thermodynamic Database for Proteins and Mutants
bullet ProtParam -Calculate aa comp, MW, pI, extinction coefficient
bullet ProtScale -Hydrophobicity and  other conformational parameters.
bullet PSIpred -Protein secondary structure prediction
bullet PSort -Prediction of signal sequence, transmembrane regions and protein localization
bullet PSort -Prediction of signal sequence, transmembrane regions and protein localization.
bullet RasMol  3D viewer
bullet SAPS -Statistical Analysis for charged clusters, repeats, hydrophobic regions, compositional domains
bullet SBASE - Protein domain library
bullet ScanProsite - Search a protein sequence against prosite pattern database
bullet Scansite - Prediction of protein signaling sequence motifs
bullet SDSC1 - Protein structure homology modeling server
bullet SignalP - Prediction of peptide signal sequence
bullet SOSUI -Prediction of Transmembrane Regions
bullet STRUCTURE -Molecular Modeling Database MMDB
bullet Swiss-Model -Automated protein modeling server
bullet Swiss-Pdb Viewer - 3D viewer
bullet TargetP -Prediction of subcellular localization
bullet TMHMM -Prediction of transmembrane helices in proteins
bullet TMpred - Prediction of membrane-spanning regions and their orientation
bullet TOPS -Protein topology cartoon generation
bullet VAST - Structure-structure similarity search
bullet WebMol - 3D viewer

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DNA / RNA  Sequence Analysis

bullet CENSOR-Query Sequence Comparison against Human/Rodent Repeats Database
bullet Gene Finder -Exon and Splice Site Prediction
bullet GENEID -Prediction of Exons and Gene Structure
bullet GenHunt -Exon Search
bullet GENIE  - Gene  Finder
bullet GenLang -Gene Structure & Transcriptional Element Features Prediction
bullet GRAIL - Exon Prediction
bullet HCpolyA - Poly-A Site Prediction
bullet HCtata -TATA Signaling Prediction
bullet HEXON - Prediction of Internal Exons in Human DNA
bullet MatInspector - Transcription Factor Binding Sites prediction
bullet McPromoter-Prediction of Transcription Start Sites in Eukaryotic DNA
bullet NNPP- Promoter Prediction
bullet NNSSP -Splice Site Prediction by Neural Network  
bullet ORD ID - Open Reading Frame search
bullet ORF Finder - Open Reading Frame Finder
bullet ORFGene -Gene Structure Prediction
bullet PatScan - DNA Sequence Motif  search
bullet Pol3Scan  -tRNA Genes Search
bullet POLYA-Search for  of 3'-end Cleavage & Poly-A Signals
bullet PROCRUSTES - Gene Recognition
bullet PYTHIA - Human Repetitive DNA Analysis
bullet RepEater  - Search for Repeats
bullet RepeatMasker2 - Search for  Repetitive Elements in DNA Sequences
bullet Signal Scan  - Search for Eukaryotic Transcriptional Elements
bullet Splice Site -Splice Site Prediction
bullet SpliceView  - Splice Signals Prediction
bullet Target Finder - Identification of  target genes of DNA-binding proteins  
bullet TESS  - Transcription Factor Binding Site Sequence Search
bullet Tfsitescan - Promoter Analysis
bullet tRNAscan  - tRNA Gene Search
bullet YSPL (BCM Gene Finder) - Exon-Exon Junction Positions Search  in cDNA

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Translation Tools
bullet Translation  Tool - Protein Translation
bullet The Protein Machine - Protein Translation
bullet 6 Frame Translation Tool - Translate DNA Sequence in all 6 Frames
bullet Reverse Translation Tools - Reverse Complement

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Primer Designing Tools

bullet Primer - Design Primers in Query Sequence
bullet Primer3-
bullet NetPrimer - Online / Offline Primer designing
bullet Primerfinder - Primer Designing Tool

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Restriction Analysis Tools

bullet WEB Cutter  -Restriction Analysis of Query Sequence
bullet ENZFINDER- Restriction analysis Tool at Molecular Biology Shortcuts
bullet Silent Restriction Sites Identification - Create a New Restriction Site Without changing the amino acid
bullet TACG- Restriction Analysis Tool

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Microarray Tools

bullet Expression Profiler- Analysis of microarray data
bullet ClustArray-DNA microarray data analysis
bullet TBI Expression Analysis -Search for  differential genes

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