Protocols:  DNA Library 

Genomic Library  
cDNA Library  
YAC Library
BAC Library  
Screening of library 

• Construction of Genome DNA Library
• Genomic Cloning Technical Manual
• Construction of Lambda DNA Library

• M13 Library Construction From Cosmids
• M13 Library Construction From BAC's or P1's)

• cDNA Library Construction  

• Bidirectional Cloning 

• cDNA Library Construction 

• Unidirectional Cloning 

YAC Library 

• Easy YAC Preparation Method  

• Preparation of YAC End Probes     
• Full Scale YAC PLUG Preparation  
• Sizing of YAC Clones  
• Subcloning of Yeast Artificial Chromosomes into Phage Lambda      
• Identification of End Clones in YAC Subclone Libraries  

Screening of Library 

• Screening of cDNA and genomic libraries  

• Screening of libraries using antibodies  

• cDNA & Genomic DNA Library Screening  

• Transfer of DNA from Bacterial Colonies to Nylon Membrane or Nitrocellulose 

• PCR based library screening

• Filter hybridization with (DIG) digoxigenin labelled probes  
• Lambda plaque screening  

• cDNA Library Screening  
• Filter hybridisation with radioactively labelled probe: Denhardt method
• Colony Hybridization  
• Colony/Plaque Blotting 
• Screening YAC libraries  

• YAC library screening by PCR on three-dimensional DNA pools  

• YAC library screening by hybridization